Part:BBa_K1140003:Design

pCons + 37 oC RNA thermometer + LacI (LVA)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 44
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

prefix: EcoRI and XbaI sufix: SpeI and PstI.

Source

Obtained by de novo synthesis. The constitutive promoter corresponds to the sequence of part BBa_J23119, the most potent member of a family of promoter variants obtained from a combinatorial library. Transcription factor LacI is the same one found in E. coli lac operon and the sequence employed is the same as the one from part BBa_C0012, which is the same as the one employed in the Elowitz and Leibler 2000 study as stated in the “design” section of the registry entry for part BBa_C0012, this LacI variant differs from the wild-type in that the GTG start present in nature was changed to an ATG start.

References

1. Elowitz MB and Leibler S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.

2. Neupert J, Karcher D, Bock R, (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. Nucleic Acids Res, 36(19):e124.